The principle of fluorescence microscope

Microscopes are a common optical instrument in the laboratory and play a vital role. There are many kinds of microscopes, of which the fluorescence microscope is one of them.

In the fluorescence microscope, excitation light of a specific wavelength must be selected in the illumination light of the specimen to generate fluorescence, and then the fluorescence in the excitation light and fluorescence must be separated from the single fluorescence to be observed. Therefore, the filter system plays an extremely important role in selecting a specific wavelength.

Principle of fluorescence microscope:

(A) Light source: The light source emits light of various wavelengths (from ultraviolet to infrared).

(B) Excitation filter light source: Pass light that can generate specific wavelengths of fluorescence for the specimen, while blocking light that is useless for exciting fluorescence.

(C) Fluorescent Specimens: Generally stained with a fluorochrome.

(D) Blocking filter: The excitation light that is not absorbed by the specimen selectively transmits fluorescence, and some of the wavelengths in the fluorescence are selectively transmitted. Ultraviolet light is used as a light source to fluoresce the illuminated object. The electron microscope was first assembled by Knorr and Haroska in Berlin in 1931. This microscope replaces the beam with a high-speed electron beam. Since the wavelength of the electron current is much shorter than the light wave, the magnification of the electron microscope can reach 800,000 times and the minimum limit of the resolution can reach 0.2 nm. Scanning electron microscopes that began to be used in 1963 allow people to see the tiny structures on the surface of objects.

Microscopes are used to magnify images of tiny objects. It is generally applied to the observation of biology, medicine, and microscopic particles.

(1) Using the movement of the micro-moving stage, with the cross eye of the full eyepiece for length measurement.

(2) Using the vernier differential angle plate of the rotating stage and the lower end of the eyepiece with the coordinates of the coordinates of the eyepiece of the eyepiece for angle measurement, so that one end of the angle to be measured is aligned with the crosshair to coincide with it, and then the other One end overlaps.

(3) Using the standard to detect the pitch, pitch diameter, outer diameter, tooth angle, and tooth shape, etc. of the thread.

(4) Examination of the crystal grain condition on the surface of the metal.

(5) Inspect the condition of the workpiece surface.

(6) Check if the size or profile of the micro-parts matches the standard.